Regulatory Elements

VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed information on these popular components, which are listed separately by category.
Enhancers
Name Description Application Notes Length Details
SV40-en Simian virus 40 enhancer Weak enhancer. 237 bp
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CMV-en Human cytomegalovirus immediate early enhancer Strong enhancer. 305 bp
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WPRE
Name Description Application Notes Length Details
WPRE Woodchuck hepatitis virus posttranscriptional regulatory element Enhances virus stability in packaging cells, leading to higher titer of packaged virus; enhances higher expression of transgenes. 598 bp
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WPRE3 Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp) Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE. 248 bp
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oPRE Optimized and truncated version of woodchuck hepatitis virus posttranscriptional regulatory element The potentially oncogenic X protein ORF and X protein promoter have been removed; ATG start sites in ORFs encoding sequences greater than 25 amino acids in length have been mutated. 581 bp
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PolyA Signals
Name Description Application Notes Length Details
BGH pA Bovine growth hormone polyadenylation signal Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. 225 bp
208 bp
225 bp
Ver 1 Ver 2 Ver 3
SV40 early pA Simian virus 40 early polyadenylation signal Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. 241 bp
135 bp
243 bp
Ver 1 Ver 2 Ver 3
SV40 late pA Simian virus 40 late polyadenylation signal Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. 222 bp
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TK pA Herpes Simplex Virus thymidine kinase polyadenylation signal Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. 272 bp
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Worm PolyA Signals
Name Description Application Notes Length Details
unc-54 3' UTR+polyA Myosin-4 3' UTR with downstream sequence from C. elegans Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. 759 bp
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tbb-2 3' UTR+polyA Tubulin beta-2 chain 3' UTR with downstream sequence from C. elegans Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. 332 bp
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