VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed information on these popular components, which are listed separately by category.
Enhancers
WPRE
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| WPRE | Woodchuck hepatitis virus posttranscriptional regulatory element | Enhances virus stability in packaging cells, leading to higher titer of packaged virus; enhances higher expression of transgenes. |
598 bp |
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| WPRE3 | Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp) | Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE. |
248 bp |
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| oPRE | Optimized and truncated version of woodchuck hepatitis virus posttranscriptional regulatory element | The potentially oncogenic X protein ORF and X protein promoter have been removed; ATG start sites in ORFs encoding sequences greater than 25 amino acids in length have been mutated. |
581 bp |
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PolyA Signals
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| BGH pA | Bovine growth hormone polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
225 bp 208 bp 225 bp |
Ver 1 Ver 2 Ver 3 |
| SV40 early pA | Simian virus 40 early polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
241 bp 135 bp 243 bp |
Ver 1 Ver 2 Ver 3 |
| SV40 late pA | Simian virus 40 late polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
222 bp |
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| TK pA | Herpes Simplex Virus thymidine kinase polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
272 bp |
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Worm PolyA Signals
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| unc-54 3' UTR+polyA | Myosin-4 3' UTR with downstream sequence from C. elegans | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. |
759 bp |
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| tbb-2 3' UTR+polyA | Tubulin beta-2 chain 3' UTR with downstream sequence from C. elegans | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. |
332 bp |
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