VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed information on these popular components, which are listed separately by category.
Selection Cassettes
| Name | Description | Application Notes | Selection Drug | References | Sequence |
|---|---|---|---|---|---|
| Neo-cassette | mPGK promoter driving Neo with SV40 late pA | Allows cells to be resistant to geneticin (G418). | Geneticin (G418) | Engineered by VectorBuilder | View |
| Puro-cassette | mPGK promoter driving Puro with SV40 late pA | Allows cells to be resistant to puromycin. | Puromycin | Engineered by VectorBuilder | View |
| LoxP-Neo-LoxP | mPGK promoter driving Neo with SV40 late pA, flanked by LoxP | Allows cells to be resistant to geneticin (G418); can be deleted by Cre recombinase. | Geneticin (G418) | Engineered by VectorBuilder | View |
| FRT-Neo-FRT | mPGK promoter driving Neo with SV40 late pA, flanked by FRT | Allows cells to be resistant to geneticin (G418); can be deleted Flp recombinase. | Geneticin (G418) | Engineered by VectorBuilder | View |
| LoxP-Puro-LoxP | mPGK promoter driving Puro with SV40 late pA, flanked by LoxP | Allows cells to be resistant to puromycin; can be deleted by Cre recombinase. | Puromycin | Engineered by VectorBuilder | View |
| FRT-Puro-FRT | mPGK promoter driving Puro with SV40 late pA, flanked by FRT | Allows cells to be resistant to puromycin; can be deleted by Flp recombinase. | Puromycin | Engineered by VectorBuilder | View |
| 3xP3-DsRed | DsRed fluorescent marker driven by 3xP3 promoter | Used for identification of genetically engineered fly lines. | - | Insect Biochem Mol Biol. 32:1221 (2002) | View |